Technologies for Detection of DNA Damage and Mutations

Technologies for Detection of DNA Damage and Mutations

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''Useful and timely.'' ---Mutagenesis ''Of considerable value.'' ---Journal of Medical Genetics ''Quite readable....a comprehensive overview....perfectly covers the needs of those researchers who have to decide on the best strategy to identify damage or mutations at the molecular level.'' ---Trends in Cell Biology ''The formats of the presentations are uniform and ample and up-to-date references are provided at the end of each chapter...will be welcomed by postgraduate researchers of all ages and should retain its usefulness for a long time.'' ---Endeavour, 21(4), 1997 This important resource thoroughly reviews a wide range of techniques used in mutagenesis research-ranging from established techniques to recently developed methodologies-based on the polymerase chain reaction. DNA damage analysis, DNA repair assays, and mutation detection are a few of the techniques featured. Chapters present detailed experimental protocols benefiting researchers and students in the fields of toxicology, biotechniques, molecular biology, photobiology, medical genetics, and oncology.Manual Instruments In our laboratory, both the Ingeny and C.B.S. manual instruments are used; each provides satisfactory results. ... The 100- to 600-bp region in the middle part of the lane (so not including the edges) is quickly cutout and applied to a 1-mmthick 10% PAAgel ... The patterns are documented under UV illumination using a Polaroid camera. ... device that comes with the automated 2-D electrophoresis instrument according to the manufacturera#39;s instructions (Ingeny B.V.).

Title:Technologies for Detection of DNA Damage and Mutations
Author: G.P. Pfeifer
Publisher:Springer Science & Business Media - 1996-07-31

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